In Vitro Evaluation Of Urolithiasis Potential For A Combination Therapy Of Aerva Lanata And Citrus Medica
Keywords:
Kidney stones,, Aerva lanata,, Citrus medica,, Calcium oxalate crystals.Abstract
The present study was to evaluate the in vitro antiurolithiatic potential of aqueous extract of Aerva lanata and aqueous extract
of Citrus medica. The objective was to assess their individual and combined efficacy in dissolving calcium oxalate crystals,
which are the primary cause of the kidney stones. Artificial kidney stone crystals were prepared by reacting calcium chloride
with sodium oxalate. Aerva lanata powder and Citrus medica powder were extracted and prepared in different concentrations.
These were tested separately and in combination with calcium oxalate crystals, and their dissolution capacity was observed under
a microscope over specified incubation periods. The highest concentration of aqueous extract of Aerva lanata (7 mg/ml)
dissolved 90% of the crystals, and aqueous extract of Citrus medica (300 mg/kg) dissolved 80%. The combination of both
extracts showed the most significant effect, dissolving 99% of the crystals in vitro. These results indicate a strong synergistic
action between the two herbal components in reducing calcium oxalate crystals. The study concludes that the combined herbal
formulation of aqueous extract of Aerva lanata and aqueous extract of Citrus medica effectively reduces calcium oxalate crystals,
indicating potential for kidney stone treatment. Further clinical trials are needed to confirm its therapeutic efficacy and safety in
humans.
